Colliform+Test


 * Period 6 Group D-Kelly Brett Julia**

Hypothesis: I expert there to be colliform bacteria in the water. I believe this because I think the water is unhealthy. However I think the tube will be about half-way filled with water because I don’t think there is an extreme amount of bacteria.

Prediction: If there is colliform bacteria in the water supply and the river we tested is unhealthy, then the water tube should be filled halfway with water and only a little colliform bacteria inside.

Procedure:

First sterilize the area with alcohol. Second, clean the top of a tube filled with Lauryl Tryptose Broth and a 5cm mini test tube inside with a lighter. Third fill a milliliter up with pond water using a pipette and put it in the tube. Forth, Clean Top with lighter again. Fifth, Flip tube over for a few seconds to make sure the 5cm mini tube fills with the liquid. Sixth, put the tube upright in an incubator for 24-48 hours.

Data: After 24 hours no oxygen has filled up in the mini test tube. In 34 degrees Celsius temperature. After 48 hours we went back to the test tube to see that it had been knocked over. The test tube. After a second try, after 24 hours there was still no oxygen. After 48, there was a bubble that was half a centimeter big.

Conclusion: In the river water that we tested, there was colliform bacteria. After research, we discovered that too much colliform means that the water is not sanitary. There was not a lot, but still some of this bacteria in the water so the river is not healthy, but defiantly not unhealthy.

Period 3, Group D: Jamie, Casey, Kaitlyn, Joe
Purpose: To see if there is colliform in the Passaic river.

Materials: Pipette, water sample, lauryl typtose broth, vial, a smaller vial to fit inside that vial, lighter, incubator, alcohol

Hypothesis: There will be some colliform in the river because there are many living organisms and they could dispose of their fecies in the river. This would give the colliform a place to live. Colliform indicates animal fecise.

Prediction: If there is colliform bacteria in the Passaic River and we do a colliform test then we should see gases in the vial showing evidence of colliform bacteria in the water.

Procedure:
 * 1) Collect water sample from the Passaic River.
 * 2) Wipe down work area with 70% alcohol.
 * 3) Open one of the tubes of sterile lauryl typtose broth.
 * 4) Flame the mouth of tube for about 3 seconds.
 * 5) Using a sterile pipette, transfer 1ml of water into the broth.
 * 6) Reflame the mouth of the tube and replace the cap.
 * 7) Turn the tube upside down to allow the vial inside to fill with broth
 * 8) Turn the tube upright
 * 9) Incubate the tube at about 35 degrees Celsius 24-48 hours
 * 10) At the end of incubation check to see if there are gasses in the vial if so how much
 * 11) Repeat steps 1 to 11 until satisfied with results

Result: Our test showed that there was one small bubble in the broth. This shows that there are small amounts of colliform bacteria in the Passaic River from where we tested from. The bubble is gas formed by the presence of colliform bacteria.

Conclusion: Our conclusion is that there are small amounts of colliform bacteria in the water. This would be a bad thing because that means there is small amounts of fecise in the water. But however small the amount is, the water is still contaminated. This could mean that only some wildlife lives very close to this area or does not drink from this specific spot. It was surprising that the bubble was so small because we took our water sample from a black pipe near the bridge close to the construction site with animal tracks. Perhaps certain species of animals don't dispose of waste in water. Maybe colliform bacteria can't survive in some bodies of water. I would say that this water is unfit to drink.


 * per.6** **Group B nicole,jacob,natalie**

Hypothesis: I believe there will be coliform bacteria because many animals excrete there fesces into the river which gives the bacteria somewhere to feed off and live off of.

Prediction:If coliform is in the water and we do an acurate coliform test than there should be oxygen in the vile which shows that coliform is present.

Procedure: 1.Collect water sample from the Passaic river at the far bridge (Central Avenue bridge) by our school. 2.Steralize area with isopropanol alchahol 70%. 3.With a lighter flame Lauryl Tryptose broth tube to steralize. 4.With a pipete take 1 ml of water and drop the water into the broth rube. 5.Flame tube again to make sure bacteria from my hand wasnt contaminating or interfiering with experiment. 6.Put cap on tight. 7.Turn tube upside down to make sure vile was filled with water. 8.Incubate at 35 degrees celsius, for 24-48 hours.

Conclusion: the first time we opened the incubator the tube didnt look like it made any progress with developing so we put the tube in for another 120 hours over the long weekend for 5 days ,Wednesday night, Thursday night, Friday night, Saturday night, and Sunday night. When we opened the incubator today ,Monday the 10th we saw particles floating in the water which shows may show signs of bacteria. Our hypothesis was correct when we said there would be bacteria on the tube. Although there were particles in the waqter, oxygen and air bubbles should fill the vile. Our hypothesis was partially correct,but there may have been some uncertaintys.We were nto satisfired with the results of the test because there were only particals in the water not actual air bubles.so this time we put the tube in again UPRIGHT and we let it sit for 48 hours the results were infact there was air bubbles resulting in a conclusion of coliform in the water. Data:The first time we did the experiment it fell over so we had to do the test again to confirm the findings. When we opened the incubator the second time we observed that there was a small bubble showing respiration or oxygen from the coliform bacteria.confirning our hypothesis. uncertaintys: The vile not being completely upright maybe had something to do with it aswell.also it was only at 34 degrees not 35 degrees.

Period 3, Group B Josh L, Julia, Johnny, Adriel Purpose : We are testing to see if there is colliform bacteria present, and if a bubble is the tube, it is there.

Hypothesis: We believe there will be a bubble present due to the respiration of the bacteria in the water sample we took from the river because that area is unhealthy.

Prediction: If the river where we took the water sample form, and we test it with the colliform test, then there should be a bubble present that is from the respiration of the bacteria.

Procedure: 1) Collect water sample. 2) Then sterilize with alcohol 3) Open one of the tubes of sterile lauryl tryptose broth. 4) With a lighter, flame the tube so it is sterilized. 5) With pipette take 1mml of water 6) Drop water into broth. 7) Put cap on tightly. 8) Turn upside down 9) Make sure it is filled with water. 10) Warm at 35 degrees Celsius for 24 to 48 hours.

Data: Our test showed a small bubble in the test tube. This proves there are some bacteria or colliform in the area of where we tested from, in our Passaic River tributary. The bubble represents the respiration of the colliform bacteria

Conclusion: We conclude that the hypothesis is supported and the water in the area we took it from is unhealthy. The bubble was there, indicating the respiration.