Petri+Dish+Test


 * Period 6: Group B-Jacob, Nicole, Natalie **

We expect that there will be harmful bacteria in the petri-dish.
 * Hypothesis:**

If there is unhealthy particles or bacteria in the petri-dish, then the river is unhealthy.
 * Prediction:**

1. Get water from river. 2. Take cotton swab and rub it all over the petri-dish. 3. Put the cover over the dish, label one side control. 4. Put petri-dish in the incubator. 5. Let dish sit there for a couple of days. 6. Take observations. 7. Count boxes on petri-dish. [|Portable Dishwasher] [|Dishwasher Reviews]
 * Procedure:**
 * Data:**
 * Day 1:** 11/14 This was the first day we put the petri-dish in the incubator. There was no bacteria in the dish.
 * Day 2:** 11/17 We had the weekend for the dish to sit in the incubator. We took it out and some water in it, also there was around 40% of bacteria in the dish. We emptied out the water out of the petri-dish.
 * Day 3:** 11/18 Today there was the same amount of bacteria as yesterday. When we took it out it smelled really bad. So obviously the petri-dish had bacteria.

_
 * Conclusion:** We found out that there was harmful bacteris in the river water. It started processing the second day we looked at the dish. The bacteria didn't grow much more than it did from when we looked at it from the second day to the third day. This shows that we were right in our hypothesis and prediction. The river is unhealthy with bacteria.

__** Period 6, Group E - Rosanna, Kara, Nick, Rich **__

__** Hypothesis: **__ __ We expect that there is harmful bacteria in the river, because we believe the water is unhealthy. __

__ ** Prediction: ** __ __ If there is a harmful bacteria in the water, and we inoculate the Petri dish with the river water, then the water must be unhealthy. __

__ ** Procedure: ** __ __ 1. Collect water from the bridge by Central Avenue. __ __ 2. Take cotton ball and rub it all over the dish (inoculating) __ __ 3. Put the cover on the dish, tape it, label it so you know what the source is __ __ 4. Put the dish in a warm environment __ __ 5. Let the dish sit for a couple of days __ __ 6. Take observations __ __ 7. We counted up all of the boxes of the experiment side of the Petri dish __ __ 8. Then we counted all of the spots on the experiment side of the Petri dish __ __ 9. For every 4 spots we counted it as 1 box. So after we count all the spots we will divide the number by 4, and that will be the number of boxes that are filled with spots. After we do that we find the percentage of how many boxes are filled up. __

__ ** Data: ** __ __ OBSERVATION 1: ** Today there is no bacteria growing yet. ** __ __ ** OBSERVATION 2: **** Today there is 54.2% bacteria growing only on the sample water side. ** __ __ ** OBSERVATION 3: **** Today there is 54.2% bacteria growing on the sample side and 0.2% on the control side. ** __

__** Conclusion: **** After doing this test can conclude that the river water is unhealthy. Not only did we get a lot of bacteria in the dish, but it was harmful bacteria. The bacteria was called cocus, it causes strep throat. Using this information we agree that the river water is unhealthy. **__ _

__ Period 6, Group C; Jocelyn,Stephanie, and Alex __ __**Hypothesis:**__ __**Prediction**__ __**Procedure:**__ __**Data:**__ __**Conclusion:**__

=Period 3, Group D Kaitlyn, Joe, Jamie, Casey=

Petri dish test
Purpose: To see if there are a lot of bacteria in the river water.

Materials: Cotton swab, Petri dish.

Hypothesis: We think that the river water is unhealthy and will have a numerous amount of bacteria. By the end of the week we should see some bacteria growth.

Procedure: 1. Collect a water sample from the river using a vial. 2. Use a cotton swab to take a small amount of water from the vial. 3. Then use the swab to inoculate half of the Petri dish using a gentle pattern. The other side will be the controlled one. 4. Put the cover on the Petri dish, label it, and tape it shut. 5. Let the dish sit in an undisturbed area. 6. Bacteria should grow in a few days. 7. Record what you see growing each week. 8. Before disposing of dish, destroy the bacteria with a small amount of house-hold bleach on the colonies while holding the dish over the sink. 9. Repeat steps until you are satisfied with your data.

Prediction: If the water is unhealthy and has a lot of bacteria and we inoculate half of a Petri dish with water from the river then we should see growth of bacteria by the end of the week.

Data:

November 12th, Day One: Today we inoculated the Petri dish. There is no bacteria growth yet. November 13th, Day Two: Very small growth of bacteria on the side of the Petri dish with water November 14th, Day Three: More bacteria grew and they are small, white and clear circles November 17th, Day Seven: Not much more bacteria grew from the weekend. The bacteria are speckled on the one side of the Petri dish and are white and clear colored.

Conclusion: Our hypothesis was supported from this data. We expected to see bacteria growth by the end of the week and after our test that is exactly what we saw. There were a lot of bacteria, but we do not know if this bacterium is bad.So further testing of the actually bacteria may be necessary.Either way though, we think that any bacteria in sufficient amount is unhealthy.So this supports our hypothesis that the river is unhealthy.


 * Period 6, Group A Kelsey, Brooke, Ian, Alex __**

**Hypothesis:** We believe that there are bacteria in the river that is affecting the cleanliness of the water in the river.

**Prediction:** If there are bacteria in the river and we inoculate the Petri dish with water and observe it each day then we should find bacteria in the Petri dish we should find unhealthy amounts of bacteria.

**Procedure:** 1. Get a Petri dish, swabs, and a sample of water from the tributary with living organism living it that water. 2. Take a swab and dip it in the 3. Fill Petri dish in half label one water sample and the other control 4. Inoculate only the half that is labeled water sample 5. Each day observe the Petri dish 6. Use bleach to kill the bacteria

**Data:**
 * Day 1:11/5/08 || 0% ||
 * Day 2:11/10/08 || 25% ||
 * Day 3:11/12/08 || 25% ||
 * Day 4:11/13/08 || 25% ||
 * Day 5:11/14/08 || 25% ||

** Conclusion: ** Our findings showed that the river has unhealthy bacteria. It also indicates that the river is unhealthy.

** Some uncertainties: ** 1)We may have counted wrong on the first time. 2)The bleach may have not killed all the bacteria 3)the living organisms could have affected our readings. 4)We didn’t test different water.

Period 3 Group E Chris Jason Ria Eleni 1. Collect a water sample from the river 2. Get a petri dish and use a cotton swab to get a small amount of water from the sample and rub it on half the petri dish 3. Draw a line on the cover a label wich side has been inoculated and wich has not (the side you did not innoculate is the control) 4. Tape the cover shut and leave in a safe place 5. Make qualative observations every day
 * Purpose:** To see if the river water is healthy or not.
 * Hypothesis:** We believe that the river water is unhealthy and that we should see some harmful bacteria grow on the petri dish.
 * Materials:** Cotton swab, water sample, petri dish, pen or pencil and notebook
 * Prediction:** If the river water is unhealthy and we innoculate half the petri dish with a water sample from the river then some harmful organisms should grow.
 * Procedure:**
 * Observations:** Small oval colonies of bacteria are dotted on the water side of the petri dish. The side with no water on it has nothing growing on it. The bacteria is a brownish tan color and has grown over night.
 * Conclusion:** Our conclusion is that the water is unhealthy because bacteria has grown over the petri dish. Our hypothesis was proven to have credibility until proven incorect. All the things we stated in our hypothesis happened. If we had more time we would have took a sample of the bacteria and studied it much more closely to find out more about the bacteria and what it means about the water's condition.